GTF2IRD1 on chromosome 7 is a novel oncogene regulating the tumor‐suppressor gene TGFβR2 in colorectal cancer

摘要

Chromosome 7q (Ch.7q) is clonally amplified in colorectal cancer (CRC). We aimed to identify oncogenes on Ch.7q that are overexpressed through DNA copy number amplification and determine the biological and clinical significance of these oncogenes in CRC. We identified general transcription factor 2I repeat domain‐containing protein 1 ( ) as a potential oncogene using a CRC dataset from The Cancer Genome Atlas with a bioinformatics approach. We measured the expression of in 98 patients with CRC using immunohistochemistry and RT‐quantitative PCR (RT‐qPCR). The biological effects of expression were explored by gene set enrichment analysis (GSEA). Next, we undertook in vitro cell proliferation and cell cycle assays using si ‐transfected CRC cells. We further investigated the oncogenic mechanisms through which promoted CRC progression. Finally, we assessed the clinical significance of expression by RT‐qPCR. was overexpressed in tumor cells and liver metastatic lesions. The GSEA revealed a positive correlation between expression and cell cycle progression‐related genes. knockdown inhibited cell proliferation and induced cell cycle arrest in ‐mutated CRC. downregulated the expression of the gene encoding transforming growth factor β receptor 2 ( ), a tumor‐suppressor gene in ‐mutated CRC. On multivariate analysis, high expression was an independent poor prognostic factor. Clinicopathological analysis showed that expression was positively correlated with liver metastasis. In conclusion, promoted CRC progression by downregulating and could be a prognostic biomarker on Ch.7q in CRC. could also be a novel oncogene in CRC.