Inhibition of connexin 43 attenuates oxidative stress and apoptosis in human umbilical vein endothelial cells

摘要

Expression of Cx43 and phospho-Cx43 after 24 h LPS stimulation. After continuous exposure to LPS for 24 h, protein levels of Cx43 in mitochondria and plasma membrane in HUVECs were detected by Western Blot ( ), which showed concentration-dependent effects from 0 to 800 ng/mL, with 800 ng/mL LPS stimulation achieving the strongest expression of Cx43 ( , ). Biotinylated Cx43 is a representation of plasma membrane Cx43. Expression of mitochondrial phospho-Cx43 was detected by Western Blot ( ), which likewise showed concentration-dependence effects from 0 to 400 ng/mL, with 400 ng/mL LPS stimulation achieving the strongest expression of phospho-Cx43 ( ). COXIV and β-actin were used as loading control. Experiments were performed in 3 biological replicates and data are presented as mean ± SD. **