Integration abundance and transmission of mutations and transgenes in a series of CRISPR/Cas9 soybean lines

摘要

Transformation vectors used in whole-plant transformations. Plant expression cassettes were integrated into the host soybean genome through -based whole-plant transformation methods using destination vectors listed on the right hand side. Promoters are blue, Cas9 endonucleases are orange, plant-selectable markers are green, and guide RNAs are pink. Different shading within each color group indicates different variants for each sequence class (e.g., the GmUbi and 35S promoters are different shades of blue)