Chromosome level assembly and comparative genome analysis confirm lager-brewing yeasts originated from a single hybridization

摘要

Structural heterozygosity within multiple copies of the chromosome I of CBS 1483. Layout of chromosome I in the assembly graph. Paths 1 and 2 (blue text and arrows) represent alternative contigs in the right-end of the chromosome—the gene is deleted in path 2. Sequencing coverage of long-read alignments of CBS 1483 in the right-end of chromosome I after joining path 1 and discarding path 2. The location of the gene is indicated. Alignment overview of five raw long-reads supporting the introgression of a ~ 14 Kbp in chromosome I (salmon colour) to a region at the right-end of chromosome XIV (brown colour) in the sub-genome. The additional alignments (pink and orange) are alignments to computationally confirmed Ty-2 repetitive elements. Schematic representation of the two chromosome architectures of chromosome XIV (brown colour) due to translocation of an additional copy of the right arm of chromosome I (salmon colour) to the left arm of chromosome XIV