Ephrin-B2 paces neuronal production in the developing neocortex

摘要

Ephrin-B2 is dynamically expressed in the developing neocortex. . in situ hybridization on transverse sections of the neocortex at different developmental stages (indicated). Scale bar: 500 μm. . Epifluorescence (GFP; green) detection on a transverse section of the neocortex of an E12.5 embryo. The section was immunostained with a phospho-EphB1–2 antibody (red) and Draq5 (blue). . Western blot analysis of E13.5 neocortex tissue extracted from control (  = 5) and (  = 6) embryos. Antibodies are indicated on the side. Quantification of the signal (bottom) shows the ratio between pEph and total Eph