DeepShape: estimating isoform-level ribosome abundance and distribution with Ribo-seq data

摘要

An example of ribosome distribution calculation on two isoforms. There are two isoforms from a gene. With existing methods that allocate multiple-mapped reads proportionally to each transcript, the central part (where reads are completely within the exon, not on the junction) of an exon in two isoforms will have the same ribosome distributions, regardless of ribosome context (e.g., the shaded area in Exon C)