PAH o-quinones produced by the Aldo-Keto-Reductases (AKRs) generate abasic sites oxidized pyrimidines and 8-oxo-dGuo via reactive oxygen species

摘要

Reactive and redox-active polycyclic aromatic hydrocarbon (PAH) o-quinones produced by Aldo-Keto Reductases (AKRs) have the potential to cause depurinating adducts leading to the formation of abasic sites and oxidative base lesions. The aldehyde reactive probe (ARP) was used to detect these lesions in calf thymus DNA treated with three PAH o-quinones (BP-7,8-dione, 7,12-DMBA-3,4-dione and BA-3,4-dione) in the absence and presence of redox-cycling conditions. In the absence of redox-cycling a modest amount of abasic sites were detected indicating the formation of a low level of covalent o-quinone depurinating adducts (> 3.2 × 10⁶ dNs). In the presence of NADPH and CuCl2, the three PAH o-quinones increased the formation of abasic sites due to ROS derived lesions destabilizing the N-glycosidic bond. The predominant source of AP sites, however, was revealed by coupling the assay with human 8-oxoguanine glycosylase (hOGG1) treatment showing that 8-oxo-dGuo was the major lesion caused by PAH o-quinones. The levels of 8-oxo-dGuo formation were independently validated by HPLC-ECD analysis. Apyrimidinic sites were also revealed by coupling the assay with Escherichia coli (Endo III) treatment showing that oxidized pyrimidines were formed but to a lesser extent. Different mechanisms were responsible for the formation of the oxidative lesions depending on whether Cu(II) or Fe(III) was used in the redox-cycling conditions. In the presence of Cu(II)-mediated PAH o-quinone redox-cycling, catalase completely suppressed the formation of the lesions but mannitol and sodium benzoate were without effect. By contrast, sodium azide, which acts as ^•OH and ¹O2 scavenger, inhibited the formation of all oxidative lesions, suggesting that the ROS responsible was ¹O2. However, in the presence of Fe(III)-mediated PAH o-quinone redox-cycling the ^•OH radical scavengers and sodium azide consistently attenuated their formation, indicating that the ROS responsible was ^•OH.