Quantitative Analysis of Trace Chromium in Blood Samples. Combination of the Advanced Oxidation Process with Catalytic Adsorptive Stripping Voltammetry

摘要

A new method for pretreating blood samples for trace Cr analysis is described. The Advanced Oxidation Process (AOP with H2O2 and 5.5-W irradiation for 60 min) is used to remove biological/organic species for subsequent analysis. Prior to the AOP pretreatment, acid (HNO3) is used at pH 3.0 to inhibit the enzyme catalase in the blood samples. Catalytic Adsorptive Stripping Voltammetry (CAdSV) at a bismuth film electrode (BiFE) gives Cr concentration of 6.0 ± 0.3 ppb in the blood samples. This concentration was confirmed by dry-ashing the blood samples and subsequent analysis by atomic absorption spectroscopy (AAS). This current method may be used to monitor chromium, a trace metal in humans, and the efficacy and safety of chromium supplements as adjuvant therapy for diabetes.