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Simultaneous Measurements of Fast Optical and Proton Current Kinetics in the Bacteriorhodopsin Photocycle using an Enhanced Spectrophotometer

机译:使用增强型分光光度计同时测量细菌视紫红质光周期中的快速光学和质子电流动力学

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摘要

A one-of-a-kind high speed optical multichannel spectrometer was designed and built at NIH and described in this journal in 1997 [Cole et al. Vol 35, pages 161–174]. The most unique aspect of this instrument was the ability to follow an entire time course from a single activation using a single sample. The instrument has been used to study rapid kinetic processes in the photon-driven bacteriorhodopsin photocycle and electron transport from cytochrome c to cytochrome aa3 and from cytochrome aa3 to oxygen. The present paper describes a second generation instrument with a number of important enhancements which significantly improve its capabilities for multichannel kinetic studies. An example application is presented in which the kinetics of photon-induced proton flow across the biological membrane is measured simultaneously with the individual steps of the photocycle determined optically. Matching the time constants for the two processes indicates which molecular transformations are associated with major proton movements.
机译:NIH设计并制造了一种独一无二的高速光学多通道光谱仪,并于1997年在该杂志上进行了描述[Cole等。第35卷,第161-174页]。该仪器最独特的方面是能够通过使用单个样品的单次激活来跟踪整个时间过程。该仪器已用于研究光子驱动的细菌视紫红质光循环中的快速动力学过程,以及电子从细胞色素c到细胞色素aa3以及从细胞色素aa3到氧气的转运。本文介绍了具有许多重要增强功能的第二代仪器,这些仪器大大提高了其用于多通道动力学研究的能力。提出了一个示例应用,其中通过光学确定光循环的各个步骤,同时测量了光子诱导的质子流经生物膜的动力学。匹配两个过程的时间常数可以表明哪些分子转化与主要质子运动有关。

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