eIF4E knockdown decreases breast cancer cell growth without activating Akt signaling

摘要

Activation of translation initiation is essential for the malignant phenotype, and is emerging as a potential therapeutic target. Translation is regulated by the expression of translation initiation factor 4E (eIF4E) as well as the interaction of eIF4E with eIF4E-binding proteins (e.g. 4E-BP1). Rapamycin inhibits translation initiation by decreasing the phosphorylation of 4E-BP1, increasing eIF4E/4E-BP1 interaction. However, rapamycin also inhibits S6K phosphorylation, leading to feedback-loop activation of Akt. We hypothesized that targeting eIF4E directly would inhibit breast cancer cell growth without activating Akt. We demonstrated that eIF4E is ubiquitously expressed in breast cancer cell lines. eIF4E knockdown by siRNA inhibited growth in different breast cancer cell subtypes including triple-negative (ER/PR/HER2-negative) cancer cells. eIF4E knockdown inhibited the growth of cells with varying total and p-4E-BP1 levels, and inhibited rapamycin-insensitive as well as sensitive cell lines. eIF4E knockdown led to a decrease in expression of cyclin D1, Bcl2 and Bcl-xL. eIF4E knockdown did not lead to Akt phosphorylation, but did decrease 4E-BP1 expression. We conclude that eIF4E is a promising target for breast cancer therapy. eIF4E-targeted therapy may be efficacious in a variety of breast cancer subtypes including triple-negative tumors for which currently there are no targeted therapies. Unlike rapamycin and its analogues, eIF4E knockdown is not associated with Akt activation.