Separation of epigallocatechin and flavonoids from Hypericum perforatum L. by high-speed counter-current chromatography and followed by preparative liquid chromatography

摘要

High-speed countercurrent chromatography (HSCCC) and preparative liquid chromatography were successively used for separation of epigallocatechin and flavonoids from Hypericum perforatum L. The two-phase solvent system composed of ethyl acetate-methanol -water (10:1:10, v/v) was used for HSCCC. About a 900 mg amount of the crude extract was separated by HSCCC, yielding 7.8 mg of quercetrin at a purity of over 97%, 12.6 mg of quercetin at a purity of over 93%, and 38.9 mg of a mixture of hyperoside, isoquercitrin and miquelianin constituting over 97% of the fraction. A mixture of epigallocatechin and avicularin pooled from three HSCCC runs, a total amount of 54.3 mg, was further separated by Prep-LC yielding 23.4 mg of epigallocatechin and 15.3 mg of avicularin each at a purity of over 97%.