TNF-induced miRNAs Regulate TNF-induced expression of E-Selectin and ICAM-1 on Human Endothelial Cells: Feedback Control of Inflammation

摘要

MicroRNAs (miRNAs) pair with target sequences in the 3’UTR of mRNAs to post-transcriptionally repress gene expression. Here we report that TNF-mediated induction of endothelial adhesion molecules can be regulated by miRNAs that are induced by TNF. Specifically, E-selectin and ICAM-1 are targets of TNF-induced miRNAs miR-31 and miR-17-3p, respectively. Specific antagonism of these TNF-induced miRNAs increased neutrophil adhesion to cultured ECs. Conversely, transfections with mimics of these miRNAs decreased neutrophil adhesion to ECs. These data suggest that miRNAs provide negative feedback control of inflammation.