The first example of MEEKC–ICP-MS coupling and its application for the analysis of anticancer platinum complexes

摘要

MEEKC is a powerful electrodriven separation technique with many applications in different disciplines, including medicinal chemistry; however the coupling to highly sensitive and selective mass spectrometric detectors was limited due to the ion suppressive effect of the surfactant SDS. Herein, the first example of the coupling of MEEKC to ICP-MS is presented and an MEEKC method for the separation of Pt(II) and Pt(IV) anticancer drugs and drug candidates was developed. Different compositions of microemulsions were evaluated and the data were compared to those collected with standard ultraviolet/visible spectroscopy (UV/vis) detection. The MEEKC–ICP-MS system was found to be more sensitive than MEEKC–UV/vis and the analysis of UV/vis silent compounds is now achievable. Furthermore, the migration behavior of the Pt(II) and Pt(IV) compounds under investigation is correlated to their differences in structure.