Expression of MicroRNA-30c and its Target Genes by nano-Bioglass Ceramic-treatment in Human Osteoblastic Cells

摘要

Osteoblast differentiation is tightly regulated by transcriptional regulators such as microRNAs (miRNAs). Several bioactive materials including nano-bioglass ceramic particles (nBGC) influence differentiation of the osteoblasts, but the molecular mechanisms of nBGC-stimulation of osteoblast differentiation via miRNAs are not yet known. In this study, we identified that nBGC-treatment stimulated expression of miR-30c in human osteoblastic cells (MG63). The bioinformatics tools identified its regulatory network, molecular function, biological process and its target genes involved in negative regulation of osteoblast differentiation. TGIF2 and HDAC4 were found to be its putative target genes and they were down regulated by nBGC-treatment in MG63 cells. Thus, this study advances our understanding of nBGC action on bone cells and supports utilization of nBGC in bone tissue engineering.