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Phytochemical Analysis and Free Radical Scavenging Activity of Medicinal Plants Gnidia glauca and Dioscorea bulbifera

机译:药用植物念珠菌和薯Di的植物化学分析和自由基清除活性

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摘要

Gnidia glauca and Dioscorea bulbifera are traditional medicinal plants that can be considered as sources of natural antioxidants. Herein we report the phytochemical analysis and free radical scavenging activity of their sequential extracts. Phenolic and flavonoid content were determined. Scavenging activity was checked against pulse radiolysis generated ABTS•+ and OH radical, in addition to DPPH, superoxide and hydroxyl radicals by biochemical methods followed by principal component analysis. G. glauca leaf extracts were rich in phenolic and flavonoid content. Ethyl acetate extract of D. bulbifera bulbs and methanol extract of G. glauca stem exhibited excellent scavenging of pulse radiolysis generated ABTS•+ radical with a second order rate constant of 2.33×106 and 1.72×106, respectively. Similarly, methanol extract of G. glauca flower and ethyl acetate extract of D. bulbifera bulb with second order rate constants of 4.48×106 and 4.46×106 were found to be potent scavengers of pulse radiolysis generated OH radical. G. glauca leaf and stem showed excellent reducing activity and free radical scavenging activity. HPTLC fingerprinting, carried out in mobile phase, chloroform: toluene: ethanol (4: 4: 1, v/v) showed presence of florescent compound at 366 nm as well as UV active compound at 254 nm. GC-TOF-MS analysis revealed the predominance of diphenyl sulfone as major compound in G. glauca. Significant levels of n-hexadecanoic acid and octadecanoic acid were also present. Diosgenin (C27H42O3) and diosgenin (3á,25R) acetate were present as major phytoconstituents in the extracts of D. bulbifera. G. glauca and D. bulbifera contain significant amounts of phytochemicals with antioxidative properties that can be exploited as a potential source for herbal remedy for oxidative stress induced diseases. These results rationalize further investigation in the potential discovery of new natural bioactive principles from these two important medicinal plants.
机译:Gnidia glauca和Dioscorea bulbifera是传统的药用植物,可以被认为是天然抗氧化剂的来源。在这里,我们报告其顺序提取物的植物化学分析和自由基清除活性。测定了酚和类黄酮的含量。通过生化方法,然后通过主成分分析,检查了脉冲辐射分解产生的ABTS •+ 和OH自由基的清除活性,以及​​DPPH,超氧化物和羟基自由基。青冈叶提取物富含酚和类黄酮。鳞茎鳞茎的乙酸乙酯提取物和青冈茎的甲醇提取物表现出优异的脉冲辐射分解产生的ABTS •+ 自由基的清除能力,其二级速率常数为2.33×10 6 和1.72×10 6 。同样地,发现青冈花的甲醇提取物和鳞茎的乙酸乙酯提取物的二阶速率常数分别为4.48×10 6 和4.46×10 6 。被脉冲辐射分解的强清除剂生成OH自由基。青冈叶和茎显示出优异的还原活性和自由基清除活性。在流动相(氯仿:甲苯:乙醇(4:4:1,v / v))中进行的HPTLC指纹图谱显示,在366 nm处存在荧光化合物,在254 nm处存在UV活性化合物。 GC-TOF-MS分析表明,二苯砜作为主要化合物存在于青冈中。也存在大量的正十六烷酸和十八烷酸。薯bulb的提取物中存在薯皂素(C27H42O3)和薯di皂素(3á,25R)作为主要植物成分。 G. glauca和D. bulbifera含有大量具有抗氧化特性的植物化学物质,可被用作氧化应激诱发疾病的草药的潜在来源。这些结果使对这两种重要药用植物新的天然生物活性成分潜在发现的进一步研究合理化。

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