Isoflavonoids from Crotalaria albida Inhibit Adipocyte Differentiation and Lipid Accumulation in 3T3-L1 Cells via Suppression of PPAR-γ Pathway

摘要

Two 2″-isopropenyl dihydrofuran isoflavonoids (>1 and >3), one 2″-isopropenyl dihydrofuran chromone (>2), as well as 13 known compounds were isolated from the herbs of Crotalaria albida. Their structures and relative configurations were elucidated via NMR and HRESIMS analyses. The 2″ S absolute configuration of >1 and >2 were deduced by comparing their NOESY spectra with that of >3, which was determined via single crystal X-ray diffraction (CuKα). The 3R absolute configuration of >1 was determined by CD. Compounds >1, >2, and >3 inhibit the adipocyte differentiation and lipid accumulation of 3T3-L1 through down-regulation of PPAR-γ activity.