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First Molecular Characterization of Feline Immunodeficiency Virus in Domestic Cats from Mainland China

机译:中国大陆家猫猫免疫缺陷病毒的第一个分子特征

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摘要

The feline immunodeficiency virus (FIV) is a retrovirus of the Lentivirus genus that was initially isolated from a colony of domestic cats in California in 1986 and has now been recognized as a common feline pathogen worldwide. To date, there is only one recent serology-based report on FIV in mainland China which was published in 2016. We designed this study to investigate the molecular prevalence and diversity of feline immunodeficiency virus (FIV) in domestic cats from mainland China. We studied the prevalence of FIV in whole blood samples of 615 domestic cats in five cities (Beijing, Guangzhou, Nanjing, Shanghai and Yangzhou) of mainland China and examined them using FRET-PCR (Fluorescence Resonance Energy Transfer-Polymerase Chain Reaction) and regular PCRs for the gag and env genes. Overall, 1.3% (8/615) of the cats were positive for provirus DNA with nucleotide analysis using PCRs for the gag and env sequences showing the cats were infected with FIV subtype A. This is the first molecular characterization of FIV in mainland China and the first description of subtype A in continental Asia.
机译:猫免疫缺陷病毒(FIV)是慢病毒属的一种逆转录病毒,该病毒最初于1986年从加利福尼亚的家猫殖民地中分离出来,现在已被公认为是全世界常见的猫病原体。迄今为止,2016年在中国大陆仅发表了一份基于血清学的关于FIV的最新报告。我们设计了这项研究,以调查中国大陆家猫中猫免疫缺陷病毒(FIV)的分子患病率和多样性。我们研究了中国大陆五个城市(北京,广州,南京,上海和扬州)的615只家猫的全血样本中FIV的患病率,并使用FRET-PCR(荧光共振能量转移-聚合酶链反应)进行了定期检查。 gag和env基因的PCR。总体上,有1.3%(8/615)的猫用gag和env序列进行的PCR核苷酸分析对原病毒DNA呈阳性,显示猫感染了FIV亚型。这是FIV在中国大陆和美国的第一个分子特征亚洲大陆对亚型A的首次描述。

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