CBMT-24. MITOCHONDRIAL DNA CONTENT IN GLIOBLASTOMA AND ITS CLINICAL SIGNIFICANCE

摘要

Evidence of altered mitochondrial DNA (mtDNA) content in several malignancies has surfaced in recent years. However, in glioblastoma, this aspect remains obscure. Hence, we have studied mtDNA content in glioblastoma at diagnosis and recurrence.METHODSFormalin fixed paraffin embedded tissue of newly diagnosed glioblastoma (n=100), paired recurrent glioblastoma (n=16 pairs) and non-neoplastic brain (n=30) archived in the department of Neuropathology, NIMHANS, were utilized for the study. Clinical details of the patients were obtained from files. IDH, ATRX and TERT promoter mutations, MGMT promoter methylation and EGFR amplification were assessed using immunohistochemistry, Sanger’s sequencing, methylation specific PCR and fluorescent insitu hybridization. mtDNA copy number was analyzed using quantitative real time PCR (relative quantification). The mtDNA content was calculated as percentage of normal using the formula 2^{- ΔΔCT} x 100. Hence, for example, 20% mtDNA copy number means that if non-neoplastic brain tissue contains 100 copies of mtDNA, the tumor contains only 20 copies.