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The potential of bacteria isolated from ruminal contents of seaweed-eating North Ronaldsay sheep to hydrolyse seaweed components and produce methane by anaerobic digestion in vitro

机译:从食用海藻的北罗纳德赛羊的瘤胃中分离出的细菌在体外通过厌氧消化水解海藻成分并产生甲烷的潜力

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摘要

The production of methane biofuel from seaweeds is limited by the hydrolysis of polysaccharides. The rumen microbiota of seaweed-eating North Ronaldsay sheep was studied for polysaccharidic bacterial isolates degrading brown-seaweed polysaccharides. Only nine isolates out of 65 utilized > 90% of the polysaccharide they were isolated on. The nine isolates (eight Prevotella spp. and one Clostridium butyricum) utilized whole Laminaria hyperborea extract and a range of seaweed polysaccharides, including alginate (seven out of nine isolates), laminarin and carboxymethylcellulose (eight out of nine isolates); while two out of nine isolates additionally hydrolysed fucoidan to some extent. Crude enzyme extracts from three of the isolates studied further had diverse glycosidases and polysaccharidase activities; particularly against laminarin and alginate (two isolates were shown to have alginate lyase activity) and notably fucoidan and carageenan (one isolate). In serial culture rumen microbiota hydrolysed a range of seaweed polysaccharides (fucoidan to a notably lesser degree) and homogenates of L. hyperborea, mixed Fucus spp. and Ascophyllum nodosum to produce methane and acetate. The rumen microbiota and isolates represent potential adjunct organisms or enzymes which may improve hydrolysis of seaweed components and thus improve the efficiency of seaweed anaerobic digestion for methane biofuel production.
机译:由海藻生产甲烷生物燃料受到多糖水解的限制。研究了食用海藻的北罗纳德赛(Non Ronaldsay)绵羊的瘤胃微生物群,以寻找降解棕褐色海藻多糖的多糖细菌分离株。 65个分离物中只有9个分离物利用了> 90%的分离多糖。九种分离物(八种普氏杆菌和一种丁酸梭菌)利用了整个海带提取物和一系列海藻多糖,包括藻酸盐(九种分离菌中的七种),层粘连蛋白和羧甲基纤维素(九种分离菌中的八种); 9个分离物中的2个在一定程度上还水解了岩藻依聚糖。进一步研究的三种分离物中的粗酶提取物具有多种糖苷酶和多糖酶活性。尤其是针对laminarin和藻酸盐(两个分离株具有藻酸盐裂解酶活性),尤其是岩藻依聚糖和角叉菜胶(一个分离株)。在系列培养中,瘤胃微生物菌群水解了一系列海藻多糖(岩藻依聚糖的含量明显降低)和高产乳杆菌的匀浆,混合了榕属菌种。和Nocosum产生甲烷和乙酸盐。瘤胃微生物群和分离物代表潜在的辅助生物或酶,它们可以改善海藻组分的水解,从而提高海藻厌氧消化甲烷生物燃料生产的效率。

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