Effect of the in vivo application of granulocyte colony‐stimulating factor on NK cells in bone marrow and peripheral blood

摘要

Granulocyte colony‐stimulating factor (G‐CSF) has been widely used in the field of allogeneic haematopoietic stem cell transplantation (allo‐HSCT) for priming donor stem cells from the bone marrow (BM) to peripheral blood (PB) to collect stem cells more conveniently. Donor‐derived natural killer (NK) cells have important antitumour functions and immune regulatory roles post‐allo‐HSCT. The aim of this study was to evaluate the effect of G‐CSF on donors' NK cells in BM and PB. The percentage of NK cells among nuclear cells and lymphocyte was significantly decreased and led to increased ratio of T and NK cells in BM and PB post‐G‐CSF in vivo application. Relative expansion of CD56^bri style="fixed-case">NK cells led to a decreased ratio of style="fixed-case">CD56^dim and style="fixed-case">CD56^bri style="fixed-case">NK subsets in style="fixed-case">BM and style="fixed-case">PB post‐G‐ style="fixed-case">CSF in vivo application. The expression of style="fixed-case">CD62L, style="fixed-case">CD54, style="fixed-case">CD94, style="fixed-case">NKP30 and style="fixed-case">CXCR4 on style="fixed-case">NK cells was significantly increased in style="fixed-case">PB after G‐ style="fixed-case">CSF treatment. G‐ style="fixed-case">CSF treatment decreased the style="fixed-case">IFN‐γ‐secreting style="fixed-case">NK population ( style="fixed-case">NK1) dramatically in style="fixed-case">BM and style="fixed-case">PB, but increased the style="fixed-case">IL‐13‐secreting style="fixed-case">NK ( style="fixed-case">NK2), style="fixed-case">TGF‐β‐secreting style="fixed-case">NK ( style="fixed-case">NK3) and style="fixed-case">IL‐10‐secreting style="fixed-case">NK ( style="fixed-case">NKr) populations significantly in style="fixed-case">BM. Clinical data demonstrated that higher doses of style="fixed-case">NK1 infused into the allograft correlated with an increased incidence of chronic graft‐vs‐host disease post‐transplantation. Taken together, our results show that the in vivo application of G‐ style="fixed-case">CSF can modulate style="fixed-case">NK subpopulations, leading to an increased ratio of T and style="fixed-case">NK cells and decreased ratio of style="fixed-case">CD56^dim and style="fixed-case">CD56^bri style="fixed-case">NK cells as well as decreased style="fixed-case">NK1 populations in both style="fixed-case">PB and style="fixed-case">BM.