A SimpleProbe® real‐time PCR assay for differentiating the canine parvovirus type 2 genotype

摘要

BackgroundCanine parvovirus type 2 (CPV‐2) causes an important canine viral disease worldwide. CPV‐2 belongs to the Protoparvovirus genus in the family Parvoviridae. An amino acid change at position 426 of the VP2 protein differentiate types of CPV‐2, designated as CPV‐2a (Asn), CPV‐2b (Asp), and CPV‐2c (Glu). In this study, we compared CPV‐2 genotyping results obtained by SimpleProbe^® real‐time PCR and DNA sequencing analysis to identify the accuracy and sensitivity of these methods.