Real-time PCR assays for quantitative detection of bovine parvovirus

摘要

In order to secure BPV safety through the present invention, a BPV detection TaqMan probe real-time PCR reaction test capable of quantitatively detecting BPV in raw materials, manufacturing processes, and finished products, and as a test method for verifying removal of BPV in the manufacturing process The law was established. In addition, primers and probes specific for BPV were selected to optimize the quantitative detection of BPV. As a result of comparing with the infecting titer by cell culture method, real - time polymerase chain reaction test showed excellent specificity and reproducibility.