Rapid and Specific Detection of Escherichia coli Sequence Type 131 (ST131) and its Key Subclones Using a Novel Single-tube Multiplex PCR Assay

摘要

Background E. coli ST131 is a recently emerged, pandemic multidrug-resistant pathogen. ST131 compromises multiple distinctive sub-clones that differ by serotype, fimH (type-1 fimbriae adhesin) allele, resistance phenotype and genotype, and host group predilection. PCR assays have been described for detecting ST131 and several of its subclones, but not all in single reaction.