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美国卫生研究院文献>Journal of Lipid Research
>Lipids in biocalcification: contrasts and similarities between intimal and medial vascular calcification and bone by NMR
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Lipids in biocalcification: contrasts and similarities between intimal and medial vascular calcification and bone by NMR
Pathomechanisms underlying vascular calcification biogenesis are still incompletely understood. Biomineral from human atherosclerotic intimal plaques; human, equine, and bovine medial vascular calcifications; and human and equine bone was released from collagenous organic matrix by sodium hydroxide/sodium hypochlorite digestion. Solid-state 13C NMR of intimal plaque mineral shows signals from cholesterol/cholesteryl esters and fatty acids. In contrast, in mineral from pure medial calcifications and bone mineral, fatty acid signals predominate. Refluxing (chloroform/methanol) intimal plaque calcifications removes the cholesterylic but not the fatty acyl signals. The lipid composition of this refluxed mineral now closely resembles that of the medial and bone mineral, which is unchanged by reflux. Thus, intimal and medial vascular calcifications and bone mineral have in common a pool of occluded mineral-entrained fatty acyl-rich lipids. This population of fatty acid may contain methyl-branched fatty acids, possibly representing lipoprotein particle remnants. Cell signaling and mechanistic parallels between physiological (orthotopic) and pathological (ectopic) calcification are also reflected thus in the NMR spectroscopic fingerprints of mineral-associated and mineral-entrained lipids. Additionally the atherosclerotic plaque mineral alone shows a significant independent pool of cholesterylic lipids. Colocalization of mineral and lipid may be coincidental, but it could also reflect an essential mechanistic component of biomineralization.
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机译:尚不清楚血管钙化生物发生的潜在机制。来自人体动脉粥样硬化内膜斑块的生物矿物;人,马和牛内侧血管钙化;通过氢氧化钠/次氯酸钠消化从胶原有机基质中释放人和马骨。内膜斑块矿物的固态 13 sup> C NMR显示来自胆固醇/胆固醇酯和脂肪酸的信号。相反,在纯钙化和骨矿物质中,脂肪酸信号占主导地位。回流(氯仿/甲醇)内膜斑钙化可去除胆甾醇,但不能去除脂肪酰基信号。现在,这种回流矿物质的脂质组成与内侧矿物质和骨骼矿物质的脂质组成非常相似,而后者在回流过程中没有变化。因此,内膜和内膜血管钙化和骨矿物质共同具有一系列被矿物质夹带的富含脂肪酰基的脂质。该脂肪酸种群可能包含甲基支链脂肪酸,可能代表脂蛋白颗粒残留物。生理性(原位)钙化和病理性(异位)钙化之间的细胞信号传导和机制相似性也反映在与矿物相关和与矿物相关的脂质的NMR光谱指纹图中。另外,单独的动脉粥样硬化斑块矿物质显示出显着的胆甾醇脂类的独立库。矿物质和脂质的共定位可能是巧合,但也可能反映了生物矿化的基本机制。
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