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Self-assembly of keratin peptides: Its implication on the performance of electrospun PVA nanofibers

机译:角蛋白肽的自组装:对电纺PVA纳米纤维性能的影响

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摘要

Drawing inspiration from the field of designer self-assembling materials, this work is aimed to focus on the self-assembling nature of extracted peptides. Hair keratin, a proteinacious reject in tanning industry has been chosen since they have been extracted and used for wide range of applications. Keratin source was subjected to five hydrolysis treatments (viz., sulphitolysis, β-mercaptoethanol, ionic liquid, thioglycolic acid and alkali) and assayed for functional groups. This was followed by the prediction of secondary structure using circular dichroism, determining the microstructural level to which the extracted peptide has self-assembled. Sulphitolysis and thioglycolic acid based hydrolysates exist in monomeric conformation, whereas β-mercaptoethanol based hydrolysate exhibited dimeric conformation. The subsequent part of the study is to incorporate these peptides into the nanofibers to study the structural implication of keratin peptides on its characteristics. Accordingly, the peptides were electrospun with PVA and subjected to morphological, mechanical, thermal and biological characterizations. Monomeric nanofiber mat has high tensile strength of around 5.5 MPa and offered lower mass transport resistance, whereas dimeric mat has high Tm of around 290 °C and was more biocompatible. These results help in understanding the extraction-structure-function aspect of the hydrolysates stressing the role of extraction methods on the choice of application.
机译:从设计师的自组装材料领域汲取灵感,这项工作旨在关注提取肽的自组装性质。头发角蛋白是制革业中一种蛋白质含量高的废品,因为它们已被提取并用于广泛的应用,因此已被选择。角蛋白源经过五次水解处理(即磺解,β-巯基乙醇,离子液体,巯基乙酸和碱),并分析了官能团。接下来是使用圆二色性预测二级结构,确定提取肽自组装的微观结构水平。硫酸酯化和基于巯基乙酸的水解物以单体构象存在,而基于β-巯基乙醇的水解物表现出二聚体构象。研究的后续部分是将这些肽掺入纳米纤维中,以研究角蛋白肽对其特征的结构影响。因此,将肽与PVA电纺丝并进行形态,机械,热和生物学表征。单体纳米纤维毡具有约5.5 MPa的高拉伸强度,并具有较低的传质阻力,而二聚体毡的Tm约为290 C,具有更高的生物相容性。这些结果有助于理解水解产物的提取结构功能方面,强调了提取方法在应用选择上的作用。

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