Evolutionary bridges to new protein folds: design of C-terminal Cro protein chameleon sequences

摘要

Regions of amino-acid sequence that are compatible with multiple folds may facilitate evolutionary transitions in protein structure. In a previous study, we described a heuristically designed chameleon sequence (SASF1, structurally ambivalent sequence fragment 1) that could adopt either of two naturally occurring conformations (α-helical or β-sheet) when incorporated as part of the C-terminal dimerization subdomain of two structurally divergent transcription factors, P22 Cro and λ Cro. Here we describe longer chameleon designs (SASF2 and SASF3) that in the case of SASF3 correspond to the full C-terminal half of the ordered region of a P22 Cro/λ Cro sequence alignment (residues 34–57). P22-SASF2 and λWDD-SASF2 show moderate thermal stability in denaturation curves monitored by circular dichroism (Tm values of 46 and 55°C, respectively), while P22-SASF3 and λWDD-SASF3 have somewhat reduced stability (Tm values of 33 and 49°C, respectively). ¹³C and ¹H NMR secondary chemical shift analysis confirms two C-terminal α-helices for P22-SASF2 (residues 36–45 and 54–57) and two C-terminal β-strands for λWDD>-SASF2 (residues 40–45 and 50–52), corresponding to secondary structure locations in the two parent sequences. Backbone relaxation data show that both chameleon sequences have a relatively well-ordered structure. Comparisons of ¹⁵N-¹H correlation spectra for SASF2 and SASF3-containing proteins strongly suggest that SASF3 retains the chameleonism of SASF2. Both Cro C-terminal conformations can be encoded in a single sequence, showing the plausibility of linking different Cro folds by smooth evolutionary transitions. The N-terminal subdomain, though largely conserved in structure, also exerts an important contextual influence on the structure of the C-terminal region.