ChopStitch: exon annotation and splice graph construction using transcriptome assembly and whole genome sequencing data

摘要

MotivationSequencing studies on non-model organisms often interrogate both genomes and transcriptomes with massive amounts of short sequences. Such studies require de novo analysis tools and techniques, when the species and closely related species lack high quality reference resources. For certain applications such as de novo annotation, information on putative exons and alternative splicing may be desirable.