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Effects of NDRG2 Overexpression on Metastatic Behaviors of HCT116 Colorectal Cancer Cell Line

机译:NDRG2过表达对HCT116结直肠癌细胞系转移行为的影响

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摘要

>Purpose: N-myc downstream-regulated gene 2 (NDRG2) is frequently down-regulated in cancer, and plays an important role in the control of tumor growth and metastasis. Its manipulation has been suggested as a therapy in cancer. Here, we examined the outcome of NDRG2 overexpression on proliferation, invasion, migration and MMP activity of HCT116 colorectal cancer cell line.>Methods: The HCT116 cell line (human colorectal cancer) was transfected with pCMV6-AC-GFP-NDRG2. 2,5diphenyltetrazolium bromide (MTT) assay was used to detect cell proliferation. The invasion and migration of the transfected cells were examined through transwell chambers while the MMP-9 activity was detected by the ability of the cells to digest gelatin.>Results: Overexpression of NDRG2 by stable NDRG2 transfection decreased cell proliferation, migration and invasion ability, along with decreasing MMP-9 activity.>Conclusion: Our data indicate that NDRG2 overexpression can suppress several aspect of tumorigenesis. Further investigations are necessitated to verify if NDRG2 molecule can be a therapeutic target in colorectal cancer.
机译:>目的:N-myc下游调节基因2(NDRG2)在癌症中经常被下调,并且在控制肿瘤的生长和转移中起着重要的作用。已经建议将其操纵作为癌症的疗法。在这里,我们检查了NDRG2过表达对HCT116结直肠癌细胞系增殖,侵袭,迁移和MMP活性的影响。>方法:用pCMV6-AC-转染HCT116细胞系(人结直肠癌)。 GFP-NDRG2。 2,5二苯基四唑溴化铵(MTT)分析用于检测细胞增殖。通过transwell小室检查转染细胞的侵袭和迁移,同时通过细胞消化明胶的能力检测MMP-9活性。>结果:稳定NDRG2转染使NDRG2过表达降低了细胞增殖>结论:我们的数据表明NDRG2过表达可以抑制肿瘤发生的多个方面。必须进行进一步的研究以验证NDRG2分子是否可以作为结直肠癌的治疗靶标。

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