Lactoferrin is an iron-binding glycoprotein found in the milk of most mammals for which various biological functions have been reported, such as antimicrobial activity and bifidogenic activity. In this study, we compared the bifidogenic activity of bovine lactoferrin (bLF) and pepsin hydrolysate of bLF (bLFH), isolated bifidogenic peptide from bLFH, and investigated the bifidogenic spectra of bLF, bLFH, and its active peptide against 42 bifidobacterial strains comprising nine species. Against Bifidobacterium breve ATCC 15700T, minimal effective concentrations of bLF and bLFH were 300 and 10 μg/ml. Against Bifidobacterium longum subsp. infantis ATCC 15697T, the minimal effective concentration of bLFH was 30 μg/ml, and bLF did not show bifidogenic activity within 300 μg/ml. As an active peptide, a heterodimer of A1-W16 and L43-A48 linked by a disulfide bond was isolated. Previously, this peptide was identified as having antibacterial activity. An amino acid mixture with the same composition as this peptide showed no bifidogenic activity. The strains of each species whose growth was highly promoted (>150%) by this peptide at 3.75 μM were as follows: B. breve (7 out of 7 strains [7/7]), B. longum subsp. infantis (5/5), Bifidobacterium bifidum (2/5), B. longum subsp. longum (1/3), Bifidobacterium adolescentis (3/6), Bifidobacterium catenulatum (1/4), Bifidobacterium pseudocatenulatum (0/4), Bifidobacterium dentium (0/5), and Bifidobacterium angulatum (0/3). Growth of none of the strains was highly promoted by bLF at 3.75 μM. We demonstrated that bLFH showed stronger bifidogenic activity than natural bLF, especially against infant-representative species, B. breve and B. longum subsp. infantis; furthermore, we isolated its active peptide. This is the first report about a bifidogenic peptide derived from bLF.
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机译:乳铁蛋白是一种在大多数哺乳动物的乳汁中发现的铁结合糖蛋白,已报道了其多种生物学功能,例如抗菌活性和双歧杆菌活性。在这项研究中,我们比较了牛乳铁蛋白(bLF)和bLF的胃蛋白酶水解物(bLFH)的双歧化活性,从bLFH中分离了双歧化肽,并研究了bLF,bLFH及其活性肽对42种双歧杆菌菌株(包括9种)的双歧化谱。种类。对于短双歧杆菌ATCC 15700 T sup>,bLF和bLFH的最低有效浓度分别为300和10μg/ ml。对抗长双歧杆菌亚种。婴儿ATCC 15697 T sup>,bLFH的最低有效浓度为30μg/ ml,在300μg/ ml范围内,bLF没有显示双歧化活性。作为活性肽,A 1 sup> -W 16 sup>和L 43 sup> -A 48 sup>的异二聚体通过分离出二硫键。以前,该肽被鉴定为具有抗菌活性。具有与该肽相同的组成的氨基酸混合物没有显示双歧化活性。每个肽的生长菌株在3.75μM下被该肽高度促进(> 150%)生长的菌株如下:短双歧杆菌(7个菌株中的7个[7/7]),长双歧杆菌亚种。婴儿(5/5),双歧双歧杆菌(2/5),长双歧杆菌长双歧杆菌(1/3),青春双歧杆菌(3/6),悬链双歧杆菌(1/4),假双歧杆菌双歧杆菌(0/4),牙双歧杆菌(0/5)和Angulatum双歧杆菌(0/3)。 3.75μM的bLF高度促进了所有菌株的生长。我们证明了bLFH表现出比天然bLF更强的双歧化活性,尤其是针对婴儿代表种B. breve和B. longum亚种。 婴儿 em>;此外,我们分离了其活性肽。这是关于源自bLF的双歧肽的首次报道。
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