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New Type of Antimicrobial Protein Produced by the Plant Pathogen Clavibacter michiganensis subsp. michiganensis

机译:植物病原体密歇根州立杆菌亚种产生的新型抗菌蛋白。密歇根州

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摘要

It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences obtained from tryptic fragments, we have identified the gene encoding CmmAMP-I and we have recombinantly produced the protein with an N-terminal intein tag. The gene sequence showed that CmmAMP-I contains a typical N-terminal signal peptide for Sec-dependent secretion. The recombinant protein was highly active, with 50% growth inhibition (IC50) of approximately 10 pmol, but was not toxic to potato leaves or tubers. CmmAMP-I does not resemble any known protein and thus represents a completely new type of bacteriocin. Due to its high antimicrobial activity and its very narrow inhibitory spectrum, CmmAMP-1 may be of interest in combating potato ring rot disease.
机译:以前已经证明番茄病原体密歇根州立细菌亚种。密歇根州分泌一种14 kDa的蛋白质,密歇根梭菌亚种。密歇根州AMP-I(CmmAMP-1),可抑制密歇根州棒状杆菌亚种的生长。 sepedonicus,马铃薯细菌性环腐的病因。使用从胰蛋白酶消化片段获得的序列,我们已经鉴定了编码CmmAMP-1的基因,并且我们重组生产了具有N末端内含肽标签的蛋白质。基因序列显示,CmmAMP-1包含一个典型的N端信号肽,用于Sec依赖性分泌。重组蛋白是高活性的,具有约10 pmol的50%生长抑制(IC50),但对马铃薯叶或块茎无毒。 CmmAMP-1与任何已知的蛋白质都不相似,因此代表了一种全新的细菌素。由于其高抗菌活性和非常窄的抑制谱,CmmAMP-1可能在对抗马铃薯环腐病中具有重要意义。

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