首页> 美国卫生研究院文献>Journal of Parasitic Diseases: Official Organ of the Indian Society for Parasitology >Comparison of microscopic and immunoassay examination in the diagnosis of intestinal protozoa of humans in Mansoura Egypt
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Comparison of microscopic and immunoassay examination in the diagnosis of intestinal protozoa of humans in Mansoura Egypt

机译:显微镜和免疫分析检查在埃及曼苏拉的人类肠道原生动物诊断中的比较

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摘要

Protozoal diseases are prevalent globally and especially in developing countries that have relatively lower socioeconomic populations such as Egypt. Direct microscopic examination (DME) is used for the detection and identification of protozoa but lacks sufficient reliability, and thus may be detrimental in obtaining accurate diagnostic or epidemiological data. In this study, we determine the prevalence of infections by Giardia intestinalis, Cryptosporidium sp., and Entamoeba histolytica in humans in Egypt. Furthermore, we determine the reliability of DME in determining infections caused by these protozoa and compare the results to enzyme linked Immunosorbent assays (ELISA). Our results indicate that the prevalence of giardiasis, cryptosporidiosis, and entamoebiasis is 38, 22, and 16 %, respectively. The sensitivity and specificity of DME for detection of G. intestinalis is 45 and 99 %, for Cryptosporidium 66 and 99 %, and for Entamoeba 45 and 100 %, respectively. Our findings demonstrate that ELISA is more reliable for diagnostic and epidemiologic study purposes.
机译:原生动物疾病在全球范围内普遍存在,尤其是在社会经济人口相对较少的发展中国家,例如埃及。直接显微镜检查(DME)用于检测和鉴定原生动物,但缺乏足够的可靠性,因此可能对获取准确的诊断或流行病学数据有害。在这项研究中,我们确定了埃及贾第鞭毛虫,隐孢子虫和溶组织变形杆菌的感染率。此外,我们确定DME在确定由这些原生动物引起的感染中的可靠性,并将结果与​​酶联免疫吸附测定(ELISA)进行比较。我们的结果表明,贾第鞭毛虫病,隐孢子虫病和肠衣病的患病率分别为38%,22%和16%。 DME检测肠杆菌的敏感性和特异性分别为45%和99%,隐孢子虫66%和99%以及Entamoeba 45%和100%。我们的发现表明,ELISA对于诊断和流行病学研究目的更为可靠。

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