A procedure for the quantitative analysis of the sulphur amino acids of rat tissues

摘要

1. A method is described for the quantitative separation of the sulphur compounds in a single sample of tissue by passing an extract through a serial assembly of ion-exchange resins in the order: Dowex 2 (Cl⁻ form), Dowex 1 (CO3²⁻ form), Amberlite CG-50 (H⁺ form) and Zeo-Karb 225 (H⁺ form). 2. Groups of sulphur amino acids were eluted separately from each column; the recovery of sulphur compounds after their labelling with ³⁵S in vivo by injection of l-[³⁵S]-methionine was 91–106%. Individual sulphur compounds were further resolved by one-dimensional or two-dimensional paper chromatography. 3. Evidence is presented on the occurrence of S-adenosylmethionine and S-adenosylhomocysteine in rat liver and brain. Rat liver and brain contained 83·6 and 31·4mμ-moles/g. respectively of S-adenosylmethionine.