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Non-thermal effects of terahertz radiation on gene expression in mouse stem cells

机译:太赫兹辐射对小鼠干细胞基因表达的非热效应

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摘要

AbstractIn recent years, terahertz radiation sources are increasingly being exploited in military and civil applications. However, only a few studies have so far been conducted to examine the biological effects associated with terahertz radiation. In this study, we evaluated the cellular response of mesenchymal mouse stem cells exposed to THz radiation. We apply low-power radiation from both a pulsed broad-band (centered at 10 THz) source and from a CW laser (2.52 THz) source. Modeling, empirical characterization, and monitoring techniques were applied to minimize the impact of radiation-induced increases in temperature. qRT-PCR was used to evaluate changes in the transcriptional activity of selected hyperthermic genes. We found that temperature increases were minimal, and that the differential expression of the investigated heat shock proteins (HSP105, HSP90, and CPR) was unaffected, while the expression of certain other genes (Adiponectin, GLUT4, and PPARG) showed clear effects of the THz irradiation after prolonged, broad-band exposure.
机译:摘要近年来,太赫兹辐射源越来越多地用于军事和民用领域。但是,到目前为止,仅进行了很少的研究来研究与太赫兹辐射有关的生物学效应。在这项研究中,我们评估了暴露于THz辐射的间充质小鼠干细胞的细胞反应。我们从脉冲宽带(以10 THz为中心)源和CW激光(2.52 THz)源中施加低功率辐射。应用了建模,经验表征和监视技术以最小化辐射引起的温度升高的影响。使用qRT-PCR评估选定的高温基因的转录活性的变化。我们发现温度升高极小,并且所研究的热激蛋白(HSP105,HSP90和CPR)的差异表达不受影响,而某些其他基因(脂联素,GLUT4和PPARG)的表达则显示了明显的作用。长时间,宽频暴露后可进行太赫兹辐射。

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