Effects of storage time and leucocyte burden of packed and buffy-coat depleted red blood cell units on red cell storage lesion

摘要

BackgroundThe red cell storage lesion (RCSL) comprises the biochemical and biomechanical changes that take place during red blood cell (RBC) storage, reducing the survival and function of these cells. Contaminating white blood cells have been major contributors to the RCSL. Markers of RCSL, such as CD47 and phosphatidylserine (PS), on RBC are attracting more attention. The aim of this study was to elucidate the effects of storage time and buffy-coat removal on CD47 and PS expression on RBC. Potassium and free haemoglobin levels in the supernatant plasma were also assessed.