Farnesylation or geranylgeranylation? Efficient assays for testing protein prenylation in vitro and in vivo

摘要

BackgroundAvailable in vitro and in vivo methods for verifying protein substrates for posttranslational modifications via farnesylation or geranylgeranylation (for example, autoradiography with ³H-labeled anchor precursors) are time consuming (weeks/months), laborious and suffer from low sensitivity.