TRAPLINE: a standardized and automated pipeline for RNA sequencing data analysis evaluation and annotation

摘要

BackgroundTechnical advances in Next Generation Sequencing (NGS) provide a means to acquire deeper insights into cellular functions. The lack of standardized and automated methodologies poses a challenge for the analysis and interpretation of RNA sequencing data. We critically compare and evaluate state-of-the-art bioinformatics approaches and present a workflow that integrates the best performing data analysis, data evaluation and annotation methods in a >Transparent, >Reproducible and >Automated >Pipe>LINE (TRAPLINE) for RNA sequencing data processing (suitable for Illumina, SOLiD and Solexa).