CRISPR/Cas9-mediated genome editing induces gene knockdown by altering the pre-mRNA splicing in mice

摘要

BackgroundClustered Regularly Interspaced Short Palindromic Repeats/CRISPR associated protein 9 (CRISPR/Cas9) has been wildly used to generate gene knockout models through inducing indels causing frame-shift. However, there are few studies concerning the post-transcript effects caused by CRISPR-mediated genome editing.