Description of a PCR-based technique for DNA splicing and mutagenesis by producing 5 overhangs with run through stop DNA synthesis utilizing Ara-C

摘要

BackgroundSplicing of DNA molecules is an important task in molecular biology that facilitates cloning, mutagenesis and creation of chimeric genes. Mutagenesis and DNA splicing techniques exist, some requiring restriction enzymes, and others utilize staggered reannealing approaches.