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Identification and Analysis of the Chloroplast rpoC1 Gene Differentially Expressed in Wild Ginseng

机译:野生人参中差异表达的叶绿体rpoC1基因的鉴定与分析

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摘要

Panax ginseng is a well-known herbal medicine in traditional Asian medicine, and wild ginseng is widely accepted to be more active than cultivated ginseng in chemoprevention. However, little has actually been reported on the difference between wild ginseng and cultivated ginseng. Thus, to identify and analyze those differences, we used suppressive subtraction hybridization (SSH) sequences with microarrays, realtime polymerase chain reaction (PCR), and reverse transcription PCRs (RT-PCRs). One of the clones isolated in this research was the chloroplast rpoC1 gene, a β subunit of RNA polymerase. Real-time RT-PCR results showed that the expression of the rpoC1 gene was significantly upregulated in wild ginseng as compared to cultivated ginseng, so, we conclude that the rpoC1 gene may be one of the important markers of wild ginseng
机译:人参是亚洲传统医学中的著名草药,在化学预防中,野山参比人参更有效。但是,关于人参与栽培人参之间的差异的报道很少。因此,为了识别和分析这些差异,我们将抑制性减法杂交(SSH)序列与微阵列,实时聚合酶链反应(PCR)和逆转录PCR(RT-PCR)结合使用。在这项研究中分离出的克隆之一是叶绿体rpoC1基因,一种RNA聚合酶的β亚基。实时RT-PCR结果表明,与栽培人参相比,rpoC1基因的表达在野生人参中明显上调,因此,我们得出结论,rpoC1基因可能是野生人参的重要标志之一。

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