首页> 美国卫生研究院文献>Journal of the Royal Society Interface >Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting
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Size and dielectric properties of skeletal stem cells change critically after enrichment and expansion from human bone marrow: consequences for microfluidic cell sorting

机译:人体干细胞富集和扩增后骨骼干细胞的大小和介电特性发生重大变化:微流细胞分选的后果

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摘要

The capacity of bone and cartilage to regenerate can be attributed to skeletal stem cells (SSCs) that reside within the bone marrow (BM). Given SSCs are rare and lack specific surface markers, antibody-based sorting has failed to deliver the cell purity required for clinical translation. Microfluidics offers new methods of isolating cells based on biophysical features including, but not limited to, size, electrical properties and stiffness. Here we report the characterization of the dielectric properties of unexpanded SSCs using single-cell microfluidic impedance cytometry (MIC). Unexpanded SSCs had a mean size of 9.0 µm; larger than the majority of BM cells. During expansion, often used to purify and increase the number of SSCs, cell size and membrane capacitance increased significantly, highlighting the importance of characterizing unaltered SSCs. In addition, MIC was used to track the osteogenic differentiation of SSCs and showed an increased membrane capacitance with differentiation. The electrical properties of primary SSCs were indistinct from other BM cells precluding its use as an isolation method. However, the current studies indicate that cell size in combination with another biophysical parameter, such as stiffness, could be used to design label-free devices for sorting SSCs with significant clinical impact.
机译:骨骼和软骨再生的能力可以归因于驻留在骨髓(BM)内的骨骼干细胞(SSC)。鉴于SSC稀少且缺乏特定的表面标记,基于抗体的分选未能提供临床翻译所需的细胞纯度。微流体技术提供了基于生物物理特征分离细胞的新方法,这些特征包括但不限于尺寸,电学性质和刚度。在这里,我们报告使用单细胞微流控阻抗细胞术(MIC)的未扩展SSCs介电特性的表征。未扩展的SSC的平均大小为9.0 µm;比大多数BM细胞大。在扩增过程中,通常用于纯化和增加SSC的数量,细胞大小和膜电容显着增加,突出了表征未改变的SSC的重要性。此外,MIC用于追踪SSC的成骨分化,并显示随着分化增加的膜电容。主要SSC的电学特性与其他BM细胞没有明显区别,因此不能用作隔离方法。但是,当前的研究表明,细胞大小与其他生物物理参数(例如刚度)的结合可用于设计无标签的设备,以对具有重大临床影响的SSC进行分类。

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