Fluorescent Activated Cell Sorting for the Characterization of Primary Rabbit Bone Marrow Fat Cell, Bone Marrow Stromal Cell, and Osteoblastic Cell Populations

摘要

As interest in the use of mesenchymal stem cell (MSC) based therapies increases, it will become important to fully characterize the cell tractions used. Several investigations have demonstrated the existence of MSCs in bone marrow stromal cells isolated from murine1 and human. Rabbits are a commonly used model for the evaluation of orthopaedic devices, but to date, bone marrow and osteogenic cell fractions from rabbit have not been characterized. We hypothesized that following monoclonal antibody labeling, fluorescent activated cell sorting (FACS) analysis could be used to characterize cell populations from rabbit bone and bone marrow stroma. Monoclonal antibodies (MAbs) for CD29, CD44, CD45, and cytokeratin were used to characterize osteoblastic, bone marrow-derived fat, and bone marrow-derived stromal cell (BMSC) fractions. Results indicated the three cell fractions were similar in composition and are a potential source of MSCs for use in the evaluation of orthopaedic cell based therapies.