Ionic mechanisms and receptor properties underlying the responses of molluscan neurones to 5-hydroxytryptamine

摘要

1. Molluscan neurones have been found to show six different types of response (three excitatory and three inhibitory) to the iontophoretic application of 5-hydroxytryptamine (5-HT). The pharmacological properties of the receptors and the ionic mechanisms associated with these responses have been analysed.2. Four of the responses to 5-HT (named A, A′, B and C) are consequent upon an increase in membrane conductance whereas the other two (named α and β) are caused by a decrease in membrane conductance.3. The A-response to 5-HT consists of a `fast' depolarization due to an increase mainly in Na<sup>+</sup>-conductance; the A′-response is a `slow' depolarization also associated with a Na⁺-conductance increase. Receptors mediating the A- and A′-depolarizations have different pharmacological properties and may exist side by side on the same neurone.4. Both the B- and C-responses are inhibitory. The B-response is a `slow' hyperpolarization due to an increase in K⁺-conductance, the C-response is a fast hyperpolarization associated with an increase in Cl^--conductance.5. The α-response to 5-HT is a depolarization which becomes reduced in amplitude with cell hyperpolarization and reverses at -75 mV; it is caused by a decrease in K⁺-conductance. The β-response is an hyperpolarization which increases in amplitude with cell hyperpolarization and reverses at -20/-30 mV. It results from a decrease in conductance to both Na⁺ and K⁺ ions.6. The receptors involved in the 5-HT responses associated with a conductance increase may be recognized by the action of specific antagonists: 7-methyltryptamine blocks only the A-receptors, 5-methoxygramine only the B-receptors and neostigmine only the C-receptors. Curare blocks the A- and C-receptors and bufotenine, the A-, A′- and B-receptors. No specific antagonists have yet been found for the 5-HT responses caused by a conductance decrease.7. The significance of the multiplicity of receptors is discussed. Their functional significance at synapses is analysed in the following paper.