Preprocessing Steps for Agilent MicroRNA Arrays: Does the Order Matter?

摘要

MOTIVATION/BACKGROUNDPrevious publications on microarray preprocessing mostly focused on method development or comparison for an individual preprocessing step. Very few, if any, focused on recommending an effective ordering of the preprocessing steps, in particular, normalization in relationship to log transformation and probe set summarization. In this study, we aim to study how the relative ordering of the preprocessing steps influences differential expression analysis for Agilent microRNA array data.