首页> 美国卫生研究院文献>Data in Brief >Differential gene expression analysis and SNP/InDel marker discovery in resistant wild Asparagus kiusianus and susceptible A. officinalis in response to Phomopsis asparagi infection
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Differential gene expression analysis and SNP/InDel marker discovery in resistant wild Asparagus kiusianus and susceptible A. officinalis in response to Phomopsis asparagi infection

机译:耐野芦笋和拟南芥对芦笋拟南芥感染的差异基因表达分析和SNP / InDel标记发现

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摘要

This data article reports de novo transcriptome analysis of resistant wild Asparagus kiusianus and susceptible A. officinalis plants 24 and 48 h post-inoculation (24 and 48 hpi) with Phomopsis asparagi. Differential gene expression (DGE) analysis demonstrated that several genes involved in secondary metabolites and plant-pathogen interactions are up-regulated in resistant wild A. kiusianus relative to susceptible A. officinalis. The assembled contig sequences generated in this study were used to search single nucleotide polymorphism (SNP) and insertion/deletion (InDel) distribution in A. kiusianus and A. officinalis plants. SNP and InDel data developed from this transcriptome analysis will be used to generate a high-density linkage map to facilitate further development of molecular marker-assisted selection in A. officinalis.
机译:该数据文章报道了接种天冬草后24和48h(24和48 hpi)的抗性野生芦笋kiusianus和敏感A.officinalis植物的从头转录组分析。差异基因表达(DGE)分析表明,相对于敏感的A. officinalis,与次生代谢产物和植物-病原体相互作用有关的几个基因在抗性野生A. kiusianus中上调。在这项研究中生成的组装重叠群序列被用来搜索A. kiusianus和A.officinalis植物中的单核苷酸多态性(SNP)和插入/缺失(InDel)分布。从该转录组分析中获得的SNP和InDel数据将用于生成高密度连锁图谱,以促进进一步开发A. officinalis中分子标记辅助选择。

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