Role of P-selectin and anti-P-selectin monoclonal antibody in apoptosis during hepatic/renal ischemia-reperfusion injury

摘要

AIM: To evaluale the potential role of P-selectin and anti-P-selectin monoclonal antibody (mAb) in apoptosis during -hepatic/renal ischemia-reperfusion injury.METHODS: Plasma P-selectin level, hepatic/renal P-selectin expression and cell apoptosis were detected in rat model of hepatic/renal ischemia-reperfusion injury. ELISA, immunohist-ochemistry and TUNEL were used. Some ischemia-reperfusion rats were treated with anti-P-selectin mAb.RESULTS: Hepatic/renal function insuffic-iency, up-regul ated expression of P-selectin in plasma and hepatic/renal tissue, hepatic/renal histopathological damages and cell apoptosis were found in rats with hepatic/renal ischemia-reperfusion injury, while these changes became less conspicuous in animals treated with anti-P-selectin mAb.CONCLUSION: P-selectin might mediate neutrophil infiltration and cell apoptosis and contribute to hepatic/renal ischemia-reperfusion injury, anti-P-selectin mAb might be an efficient approach for the prevention and treatment of hepatic/renal ischemia-reperfusion injury.