Antitumor Effects of DC Vaccine With ALA-PDT-Induced Immunogenic ApoptoticCells for Skin Squamous Cell Carcinoma in Mice

摘要

Targeted immunotherapy using dendritic cell vaccine has been employed for the treatment of solid tumors. Topical 5-aminolevulinic acid-mediated photodynamic therapy, an established approach for topical cancers, can induce an effective antitumor immune response. We have previously shown that 5-aminolevulinic acid-mediated photodynamic therapy–induced tumor lysates could considerably enhance antigen-presenting capacity of ex vivo-generated dendritic cells. The current study further demonstrates that 5-aminolevulinic acid-mediated photodynamic therapy dendritic cell vaccine can induce immune responses against cancers. Dendritic cells pulsed by photodynamic therapy–treated skin squamous cell carcinoma cells inhibited squamous cell carcinoma to a greater extent than tumor lysates treated by photodynamic therapy alone or dendritic cells pulsed by freeze–thawed treated tumor cells. Immunohistochemistry showed that photodynamic therapy dendritic cell vaccine could increase the activity of CD4⁺ and CD8⁺ T cells in the tumor implantation sites. Flow cytometry assays showed that CD4⁺ and CD8⁺ T cells in the spleens of photodynamic therapy dendritic cell vaccine immunized mice increased significantly. Furthermore, we observed increased amounts of interleukin 12 and Interferon gamma (IFN-γ) and decreased amounts of interleukin 10 in the splenocytes and peripheral blood ofphotodynamic therapy dendritic cell vaccine immunized mice by enzyme linked immunosorbentassay (ELISA). Taken together, our findings suggest that photodynamic therapy dendriticcell vaccination is an effective prophylactic therapy for squamous cell carcinoma.