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Electroanalytical Sensors and Devices for Multiplexed Detection of Foodborne Pathogen Microorganisms

机译:用于食源性病原微生物多重检测的电分析传感器和设备

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摘要

The detection and identification of pathogen microorganisms still rely on conventional culturing techniques, which are not suitable for on-site monitoring. Therefore, a great research challenge in this field is focused on the need to develop rapid, reliable, specific, and sensitive methods to detect these bacteria at low cost. Moreover, the growing interest in biochip development for large scale screening analysis implies improved miniaturization, reduction of analysis time and cost, and multi-analyte detection, which has nowadays become a crucial challenge. This paper reviews multiplexed foodborne pathogen microorganisms detection methods based on electrochemical sensors incorporating microarrays and other platforms. These devices usually involve antibody-antigen and DNA hybridization specific interactions, although other approaches such as the monitoring of oxygen consumption are also considered.
机译:病原微生物的检测和鉴定仍然依赖于常规培养技术,这些技术不适用于现场监测。因此,在该领域中的巨大研究挑战集中在需要开发快速,可靠,特异性和灵敏的方法以低成本检测这些细菌的需求。此外,对用于大规模筛选分析的生物芯片开发的兴趣日益增长,这意味着改进的小型化,减少分析时间和成本以及进行多分析物检测,这已成为当今的关键挑战。本文综述了基于结合了微阵列和其他平台的电化学传感器的食源性病原微生物的多重检测方法。这些设备通常涉及抗体-抗原和DNA杂交的特异性相互作用,尽管还考虑了其​​他方法,例如监测耗氧量。

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