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美国卫生研究院文献>The Scientific World Journal
>Optimization of Acid Protease Production by Aspergillus niger I1 on Shrimp Peptone Using Statistical Experimental Design
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Optimization of Acid Protease Production by Aspergillus niger I1 on Shrimp Peptone Using Statistical Experimental Design
Medium composition and culture conditions for the acid protease production by Aspergillus niger I1 were optimized by response surface methodology (RSM). A significant influence of temperature, KH2PO4, and initial pH on the protease production was evaluated by Plackett-Burman design (PBD). These factors were further optimized using Box-Behnken design and RSM. Under the proposed optimized conditions, the experimental protease production (183.13 U mL−1) closely matched the yield predicted by the statistical model (172.57 U mL−1) with R 2 = 0.914. Compared with the initial M1 medium on which protease production was 43.13 U mL−1, a successful and significant improvement by 4.25 folds was achieved in the optimized medium containing (g/L): hulled grain of wheat (HGW) 5.0; KH2PO4 1.0; NaCl 0.3; MgSO4(7H2O) 0.5; CaCl2 (7H2O) 0.4; ZnSO4 0.1; Na2HPO4 1.6; shrimp peptone (SP) 1.0. The pH was adjusted at 5 and the temperature at 30°C. More interestingly, the optimization was accomplished using two cheap and local fermentation substrates, HGW and SP, which may result in a significant reduction in the cost of medium constituents.
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机译:通过响应面法(RSM)优化了黑曲霉I1产生酸性蛋白酶的培养基组成和培养条件。通过Plackett-Burman设计(PBD)评估了温度,KH2PO4和初始pH对蛋白酶产生的显着影响。使用Box-Behnken设计和RSM进一步优化了这些因素。在建议的优化条件下,实验蛋白酶的产量(183.13 U mL -1 sup>)与统计模型(172.57 U mL -1 sup>)预测的收率紧密匹配,R < sup> 2 sup> = 0.914。与最初的M1培养基的蛋白酶产量为43.13 U mL -1 sup>相比,在含有(g / L)的优化的培养基中,成功地显着提高了4.25倍: HGW)5.0; KH2PO4 1.0;氯化钠0.3; MgSO4(7H2O)0.5; CaCl2(7H2O)0.4;硫酸锌0.1; Na2HPO4 1.6;虾蛋白ept(SP)1.0。将pH调节至5,并将温度调节至30℃。更有趣的是,使用两种廉价的本地发酵底物HGW和SP进行了优化,这可能导致培养基成分的成本大大降低。
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