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Mass spectrometrical analysis of recombinant human growth hormone (Genotropin®) reveals amino acid substitutions in 2 of the expressed protein

机译：重组人类生长激素（Genotropin®）的质谱分析显示2％的表达蛋白质具有氨基酸取代

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BackgroundThe structural integrity of recombinant proteins is of critical importance to their application as clinical treatments. Recombinant growth hormone preparations have been examined by several methodologies. In this study recombinant human growth hormone (rhGH; Genotropin^®), expressed in E. coli K12, was structurally analyzed by two-dimensional gel electrophoresis and MALDI-TOF-TOF, LC-MS and LC-MS/ MS sequencing of the resolved peptides.

机译：背景技术重组蛋白的结构完整性对其作为临床治疗的应用至关重要。重组生长激素制剂已通过几种方法进行了研究。在这项研究中，重组人生长激素（rhGH; Genotropin ^{®）在大肠杆菌K12中表达，通过二维凝胶电泳，MALDI-TOF-TOF，LC-MS和LC-分离肽的MS / MS测序。}

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期刊名称 Proteome Science
作者
Felix Hepner; Edina Cszasar; Elisabeth Roitinger; Gert Lubec;
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年(卷),期 2005(3),-1
年度 2005
页码 1
总页数 12
原文格式 PDF
正文语种
中图分类生化遗传学;生化药理学;
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专利

1. Mass spectrometrical analysis of recombinant human growth hormone (Genotropin?) reveals amino acid substitutions in 2% of the expressed protein [J] . Felix Hepner, Edina Cszasar, Elisabeth Roitinger, Proteome science . 2005,第2期

机译：重组人类生长激素（Genotropin ？）的质谱分析显示2％的表达蛋白质具有氨基酸取代
2. Massspectrometrical analysis of recombinant human growth hormone Norditropin reveals amino acid exchange at M14_V14 rhGH. [J] . Hepner F, Csaszar E, Roitinger E, Proteomics . 2006,第3期

机译：重组人生长激素Norditropin的质谱分析揭示了M14_V14 rhGH的氨基酸交换。
3. Mass Spectrometric Analysis of Innovator,Counterfeit,and Follow-On Recombinant Human Growth Hormone [J] . Haitao Jiang, Shiaw-Lin Wu, Barry L.Karger Biotechnology Progress . 2009,第1期

机译：创新者，假冒和后续重组人类生长激素的质谱分析
4. Comprehensive Mass Spectrometric Analysis of Multiple Pertussis Toxins Reveals Amino Acid Homogeneity and Differential Subunit Expression [C] . Yulanda M. Williamson, Hercules Moura, David Schieltz, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2009

机译：综合质谱分析多个百日咳毒素揭示氨基酸均匀性和差异亚基表达
5. Mass spectrometric studies on glycoprotein oligosaccharides: A modified procedure for the liquid secondary ion mass spectrometric analysis of glycoprotein oligosaccharides. Studies on the nature of glycosylation on baculovirus-expressed mouse interleukin-3. [D] . Hogeland, Kenneth Eden, Jr. 1993

机译：糖蛋白寡糖的质谱研究：糖蛋白寡糖的液体二次离子质谱分析的改良程序。杆状病毒表达的小鼠白介素-3糖基化性质的研究。
6. Mass Spectrometry Analysis Coupled with de novo Sequencing Reveals Amino Acid Substitutions in Nucleocapsid Protein from Influenza A Virus [O] . Zijian Li, Wanchun Sun, Donglin Wu, 2014

机译：质谱分析与从头测序相结合揭示了甲型流感病毒核壳蛋白中的氨基酸取代
7. Mass spectrometrical analysis of recombinant human growth hormone (Genotropin^®) reveals amino acid substitutions in 2% of the expressed protein [O] . Roitinger Elisabeth, Cszasar Edina, Hepner Felix, 2005

机译：重组人类生长激素（Genotropin ^{®）的质谱分析显示2％的表达蛋白质具有氨基酸取代}

Mass spectrometrical analysis of recombinant human growth hormone (Genotropin®) reveals amino acid substitutions in 2 of the expressed protein

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