Distinct roles for IL-13 and IL-4 via IL-13 receptor α1 and the type II IL-4 receptor in asthma pathogenesis

摘要

IL-13 and IL-4 are central T helper 2 (Th2) cytokines in the immune system and potent activators of inflammatory responses and fibrosis during Th2 inflammation. Recent studies using Il13ra1^−/− mice have demonstrated a critical role for IL-13 receptor (IL-13R) α1 in allergen-induced airway responses. However, these observations require further attention especially because IL-4 can induce similar lung pathology to IL-13, independent of IL-13, and is still present in the allergic lung. Thus, we hypothesized that IL-13Rα1 regulates IL-4-induced responses in the lung. To dissect the role of IL-13Rα1 and the type I and II IL-4Rs in experimental asthma, we examined lung pathology induced by allergen, IL-4, and IL-13 challenge in Il13ra1^−/− mice. We report that IL-13Rα1 is essential for baseline IgE production, but Th2 and IgE responses to T cell-dependent antigens are IL-13Rα1-independent. Furthermore, we demonstrate that increased airway resistance, mucus, TGF-β, and eotaxin(s) production, but not cellular infiltration, are critically dependent on IL-13Rα1. Surprisingly, our results identify a CCR3- and IL-13Rα1-independent pathway for lung eosinophilia. Global expression profiling of lungs from mice stimulated with allergen or IL-4 demonstrated that marker genes of alternatively activated macrophages are differentially regulated by the type I and type II IL-4R. Taken together, our data provide a comprehensive mechanistic analysis of the critical role by which IL-13Rα1 mediates allergic lung pathology and highlight unforeseen roles for the type II IL-4R.